Volume 5, Issue 5, September 2019, Page: 61-67
Microscopic Studies, Phytochemical and Biological Screenings of Ocimum canum
Dorothée Dinangayi Tshilanda, Department of Chemistry, Faculty of Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Clément Liyongo Inkoto, Department of Nutrition, Public Health School, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Kashala Mpongu, Department of Chemistry, Faculty of Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Souzane Mata, Department of Biology, Faculty of Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Paulin Kapepula Mutwale, Centre d’Etudes des Substances Naturelles d’Origine Végétale (CESNOV), Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Damien Sha-Tshibey Tshibangu, Department of Chemistry, Faculty of Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Gédéon Ngiala Bongo, Department of Biology, Faculty of Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Ngbolua Koto-te-Nyiwa, Department of Biology, Faculty of Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Pius Tshimankinda Mpiana, Department of Chemistry, Faculty of Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Received: Aug. 15, 2019;       Accepted: Oct. 7, 2019;       Published: Oct. 25, 2019
DOI: 10.11648/j.ijpc.20190505.13      View  26      Downloads  14
Abstract
It has been reported that more than 80% of the African population uses traditional medicine to primary healthcare. The aim of the study was to determine the histological elements, the phytochemical composition and evaluate the bioactivities of Ocimum canum in order to promote this plant used in Congolese traditional medicine. The earthworms were collected from the ponds of the secondary forest of Monastère Prieure Notre Dame de l’Assomption in Kinshasa city and the blood used in this study was provided by the Centre de Médecine Mixte et d’Anémie SS in Yolo-Sud quarter, Kinshasa, Democratic Republic of the Congo. The histological elements were determined by microscopic examination while different metabolites were determined using thin layer chromatography. Microscopic examination revealed the presence of crystalliferous fibres, fragment of parenchyma, grandular and non-glandular pluricellular trichomes, spheroidal pollen grains, diacytic stomata, spiral vessels. With regard to chromatographic analysis, phytochemicals identified were anthocyanins, coumarins, flavonoids, terpenoids and iridoids. The extracts from O. canum showed good antihelminthic, antisickling and antioxidant activities related to their phytochemicalsAll these findings constitute a scientific evidence validating the use of this medicinal plant for the management of various ailments in the Democratic Republic of the Congo.
Keywords
Antihelminthic Activity, Microscopic Features, Oleanolic Acid, Ocimum canum, Rosmarinic Acid
To cite this article
Dorothée Dinangayi Tshilanda, Clément Liyongo Inkoto, Kashala Mpongu, Souzane Mata, Paulin Kapepula Mutwale, Damien Sha-Tshibey Tshibangu, Gédéon Ngiala Bongo, Ngbolua Koto-te-Nyiwa, Pius Tshimankinda Mpiana, Microscopic Studies, Phytochemical and Biological Screenings of Ocimum canum, International Journal of Pharmacy and Chemistry. Vol. 5, No. 5, 2019, pp. 61-67. doi: 10.11648/j.ijpc.20190505.13
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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