Phytochemical Analysis and Bioactivities Studies of Fresh Leaves and Flowers from C. roseus, L. multiflora and P. amarus, Beninese Medicinal Plants Used Against Diabetes
Simplice Koudjina,
Alban Gouton Houngbeme,
Israël Dossou Paulin Agbogba,
Guy Yacolé Sylvain Atohoun,
Joachim Djimon Gbenou
Issue:
Volume 9, Issue 5, September 2023
Pages:
56-66
Received:
Aug. 30, 2023
Accepted:
Sep. 21, 2023
Published:
Oct. 08, 2023
Abstract: Traditional herbal medicines make up a large part of drug use. This work addressed an area of public health, the fight against diabetes. The present study aimed to carry out qualitative and quantitative screening of the secondary metabolites of extracts from the leaves and flowers of plants such as Catharanthus roseus, Lippia multiflora and Phyllanthus amarus, and to evaluate the antioxidant activity in their aqueous and ethanolic extracts, their larval toxicity and then to predict the antidiabetic activity. The results showed that its plants have in their various extracts some secondary metabolites such as alkaloids, tannins, flavonoids, coumarins, terpenoids, phenolic compounds, steroids, etc responsible for several interesting pharmaceutical activities. These medicinal plants showed a higher quantity of polyphenols in the hydro-ethanolic extracts, with levels of 60.65±0.13; 44.40±1.43 and 67.11±0.39 mg.eq/g respectively for C. roseus, L. multiflora and P. amarus and also a higher antioxidant potential for L. multiflora and P. amarus than the standard Vitamin C, with IC50 equal to 0.53 and 0.52 mg/mL respectively. Extracts are not toxic against larvae. These results are an important indicator of the hypoglycemic and anti-diabetic activity and confirmed the potential for use of these plants by the population in the fight against diabetes.
Abstract: Traditional herbal medicines make up a large part of drug use. This work addressed an area of public health, the fight against diabetes. The present study aimed to carry out qualitative and quantitative screening of the secondary metabolites of extracts from the leaves and flowers of plants such as Catharanthus roseus, Lippia multiflora and Phyllan...
Show More
Research Article
Determination of Analytical Parameters of Some 2-arylvinyl Benzimidazole Derivatives by Liquid Chromatography Coupled with Tandem Mass Spectrometry (LC/MS/MS)
Nina Carine Ablé,
Deto Ursul Jean-Paul N’guessan,
Mariette Désirée Yéhé,
Songuigama Coulibaly,
Jean-Kisito Kouamé*,
Claude Bérenger Ngalemo Ngantchouko,
Laurent Kipré Séri,
Aïsséta Kourouma,
Gildas Komenan Gbassi
Issue:
Volume 9, Issue 5, September 2023
Pages:
67-77
Received:
Oct. 25, 2023
Accepted:
Nov. 13, 2023
Published:
Nov. 21, 2023
DOI:
10.11648/j.ijpc.20230905.12
Downloads:
Views:
Abstract: The Benzimidazole nucleus is a heterocyclic with many pharmacological activities due to its pharmacophore power. Given the specific nature of this nucleus, researchers and the pharmaceutical industry are trying to develop new molecules based on it in order to broaden the therapeutic arsenal for recurring diseases throughout the world, and particularly in Africa. The present study aimed to identify and characterize some derivatives of 2-arylvinyl Benzimidazole synthesized by chemists from the Department of medicinal chemistry and Organic Chemistry of the Training and Research Unit of Pharmaceutical and Biological Sciences of the Felix Houphouët Boigny University from Abidjan (Ivory Coast). The technique used to carry out the study was liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). The ZORBAX Eclipse plus C18 type column (2.1 X 50 mm-1.8 µm) was used for the chromatographic separation of the compounds. The majority of compounds were eluted isocratically in the proportions (80%:20%) for ten compounds, (90%:10%) for eight compounds and (75%:25%) for one compound. The mobile phase used to elute the compounds consisted of: (Water + Formic Acid (0.1%) / Acetonitrile + Formic Acid (0.1%)). The elution flow rates were set at 0.30 and 0.45 mL/min and the injection volume was set at 3 µL to separate most of the compounds. The ms/ms detector parameters corresponding to each of these derivatives were also determined and used to deduce the retention time (rt) and mass spectrum (m/z) of each of these derivatives. Of these, eighteen compounds had theoretical molecular weights (Theoretical MW) that were close to their experimental molecular weights (Experimental MW), unlike one compound. Analytical parameters (chromatographic (LC) and ms/ms) were therefore used to identify and characterize the various synthesis products that were the subject of this study.
Abstract: The Benzimidazole nucleus is a heterocyclic with many pharmacological activities due to its pharmacophore power. Given the specific nature of this nucleus, researchers and the pharmaceutical industry are trying to develop new molecules based on it in order to broaden the therapeutic arsenal for recurring diseases throughout the world, and particula...
Show More
