The Cytotoxicity of Analogues Carnosine Dipeptide on Human Glioblastoma Multiforme
Mohammadreza Gholibeikian,
Nastaran Gholami Samali,
Amirreza Arvaneh
Issue:
Volume 8, Issue 6, November 2022
Pages:
67-74
Received:
8 October 2022
Accepted:
10 November 2022
Published:
13 January 2023
Abstract: Background & Aims: The present study evaluated the effect of some synthesized linear and cyclic Carnosine analogues on the cells of human glioblastoma multiforme (GBM). The purpose of this research was to study the exposure of Carnosin analogues on astrocytoma using several experiments. Methods: The mass spectral measurements were performed on a 6410 Agilent LCMS triple quadrupole mass spectrometer (LCMS) with an electrospray ionization (ESI) interface. All tests were carried out six times. The concentration used for peptides (10 µg/mL) was selected based on MTT assay. The effect of peptides on the activity of SDH was assayed by MTT test. 100 μL mitochondrial suspension from GBM and normal groups were incubated with applied concentration of peptides (10μg/mL) at 37°C for 30 min. DCFH (final concentration, 10µM) was added to cells of both groups and incubated at 37°C for 15 min. The fluorescence intensity of DCFH which is an indicator of ROS concentration was then assayed by a Shimadzu RF-5000U fluorescence spectrophotometer. The cationic fluorescent dye, rhodamine 123 (concentration10 µM), from mitochondria into the cytosol have been used for the determination of MMP collapse. Mitochondria from astrocytoma and normal astocyte groups were suspended in corresponding assay buffer and incubated at 37°C with 10µg/mL peptides. The release was assayed by the Quantikine Cytochrome c. Carnosine peptide analogues on the activation of caspase-3 in the mitochondria isolated from GBM and normal groups using Sigma’s caspase-3 colorimetric assay kit. Results: Our study showed that a various range of toxicity on GBM cells was resulted by the linear and cyclic Carnosine analogues using MTT assay. Also, applying peptides on the mitochondria of GBM cells, a raise of mitochondrial reactive oxygen species (ROS) level, mitochondrial swelling, mitochondrial membrane potential (∆ψm) collapse, release of cytochrome c and caspase-3 activation of the affected mitochondria were detected. Conclusion: Based on the overall results, cyclic Carnosine analogues, especially compound 1c [Cyclo-(β-alanine-¬His-Pro-¬β-alanine-¬His)] showed more toxic activity than linear Carnosine analogues, which would be supporting to develop Carnosine cyclic peptide analogues as new anticancer and complementary therapeutic agents for the treatment of glioblastoma.
Abstract: Background & Aims: The present study evaluated the effect of some synthesized linear and cyclic Carnosine analogues on the cells of human glioblastoma multiforme (GBM). The purpose of this research was to study the exposure of Carnosin analogues on astrocytoma using several experiments. Methods: The mass spectral measurements were performed on a 64...
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Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae)
Claude Berenger Ngantchouko Ngalemo,
Gisele Laurel Tchiengang Tchoua,
Francois Nicaise Bony,
Blondin Gatien Tsawo,
Patrick Yamen Mbopi,
Stephane Kevin Ndengue Langoul,
Jean Michel Tekam,
Pierre Rene Fotsing Kwetche
Issue:
Volume 8, Issue 6, November 2022
Pages:
75-81
Received:
29 December 2022
Accepted:
16 January 2023
Published:
31 January 2023
Abstract: Erectile dysfunction has become for two decades, a real public health problem. Its prevalence is estimated at 322 million in 2025. Its management is based on the administration of phosphodiesterase inhibitors alone or in combination. Infections have been rarely mentioned although E coli, Staphylococcus aureus, Klebsiella pneumoniae or Serratia marcescens are recognized as factors of erectile dysfunction. The purpose of this study was to evaluate the phytochemical profile of the aqueous (AE) and hydroethanolic (HE) extracts of the fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. The aim of this study was to evaluate phytochemical profile of aqueous (AE) and hydroethanolic (HE) extracts of fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. Phytochemical profile highlighted the presence of alkaloids, polyphenols with concentrations of 434.5 ± 14.5 mgEq Tannic acid/g of dried extract greater than 249.5±18.3 mgEq Tannic acid/g of dried extract found in AE including flavonoids whose concentrations is 184.9 ± 6.3 μgEqRutin/g of dry extract in HE against 183.0±9.1 μgEqRutin/g of dried extract in AE. Antibacterial tests showed equivalence of activity on E coli and S aureus strains with MICs of 100 mg/mL and 50mg/mL respectively and only HE showed activity on Klebsiella pneumoniae and Serratia marcescens which had the lowest MIC 6.25 mg/mL.
Abstract: Erectile dysfunction has become for two decades, a real public health problem. Its prevalence is estimated at 322 million in 2025. Its management is based on the administration of phosphodiesterase inhibitors alone or in combination. Infections have been rarely mentioned although E coli, Staphylococcus aureus, Klebsiella pneumoniae or Serratia marc...
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